Fig. 2

Effect of B7-H4 on abnormal pregnancy outcomes caused by T. gondii infection in pregnant female mice. a WT uninfected pregnant female mice had good mental states, bright hair, and well-developed placentas and fetuses. b WT infected pregnant female mice had poor mental conditions, dull hair, and small placentas and fetuses with poor blood supply. c B7-H4^−/− infected pregnant female mice were in a poor mental state with dull hair and dim eyes, and had more stillbirths and absorptive fetuses. d The weight of the placenta and fetus and the resorption rate in WT uninfected, WT infected, and B7-H4^−/− infected pregnant female mice. e Representative scanning electron microscopy (SEM) images of the fetal development showing fetal size, eyeballs, fingers, and toes. f Representative hematoxylin and eosin (H&E) staining images of placentas from WT uninfected, WT infected, and B7-H4^−/− infected pregnant female mice, showing fibrinoid necrosis of blood vessels (black circle). Data shown are means ± SD. At least eight pregnant female mice in each group were assayed individually by unpaired t-test for uninfected vs infected group, infected vs B7-H4^−/−infected group