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Fig. 5 | Parasites & Vectors

Fig. 5

From: B7-H4 reduction induced by Toxoplasma gondii infection results in dysfunction of decidual dendritic cells by regulating the JAK2/STAT3 pathway

Fig. 5

Reduction of B7-H4 on dDCs by T. gondii infection associated with the expression of the intracellular enzyme IDO. a Flow cytometry analyses of B7-H4 and IDO levels in CD11c+CD8a and CD11c+CD8a+ dDCs from WT uninfected, WT infected, and B7-H4−/− infected groups of pregnant female mice. b Histogram analysis of B7-H4 and IDO expression changes in CD11c+CD8a and CD11c+CD8a+ dDCs from WT uninfected, WT infected, and B7-H4−/− infected pregnant female mice. c Representative images of IF of IDO (green) expression levels in purified human dDCs from uninfected, infected, and B7-H4-neutralized infected pregnant female mice. DAPI (blue) labeling defined the morphology of nuclei. Scale bars, 10 μm. d Histogram analysis of IDO expression changes on purified human dDCs by immunofluorescence intensity among three groups. Data shown are means ± SD. At least six samples of human decidua in each group were assayed individually in flow cytometry and at least three samples of human decidua in each group were assayed individually in Western blot and IF by paired t-test for uninfected vs infected group, infected vs B7-H4 neutralized infected group; At least eight pregnant female mice in each group were assayed individually by unpaired t-test for uninfected vs infected group, infected vs B7-H4−/− infected group

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