Fig. 7

Reduction of B7-H4 on dDCs by T. gondii infection associated with the expression of IDO, IL-10, and IL-12 via regulation of the JAK2/STAT3 pathway. a, c Western blotting assay of B7-H4, IDO, IL-10, IL-12, JAK2, p-JAK2, STAT3, and p-STAT3 levels from uninfected, infected, and B7-H4-neutralized infected groups of purified human dDCs. b, d Western blotting assay analysis of B7-H4, IDO, IL-10, IL-12, STAT3, and p-STAT3 levels from uninfected, infected, STAT3 inhibitor-treated, or STAT3 inhibitor-treated infected groups of purified human dDCs. Data shown are means ± SD. At least three samples of human decidua in each group were assayed individually by paired t-test in Western blot; uninfected vs infected group, infected and B7-H4-neutralized infected group. e A pattern diagram of possible mechanisms by which T. gondii infection can lead to a decrease in B7-H4 on the dDC, ultimately leading to dysfunction of functional molecule