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Table 2 Prevalence, assemblages, sub-assemblages and MLGs of G. duodenalis in different animals in Heilongjiang Province

From: Giardia duodenalis in patients with diarrhea and various animals in northeastern China: prevalence and multilocus genetic characterization

Hosta

Positive no./examined no. (%)

Assemblage (n)

Sub-assemblage (n)/accession no.

MLGs (n)

SSU rRNA

bg

tpic

gdh

Cattle (Bos taurus)

47/138 (34.06)

D (1)

D (1)/OM001828

 

D1 (1)/OM001863

 

E (46)

E1 (1)/OM001829

E1  (1)/OM001847

E1 (1)/OM001865

MLG-E1 (1)

 

B2 (1), E1 (4), E4 (1)/OM001850, E6 (1), AI-1/E1 (1)

  

E2 (1)/OM001830

E2 (1)/OM001848

  

E3 (1)/OM001831

E3 (1)/OM001849

E1 (1)

MLG-E2 (1)

E2 (1), E4 (1)/OM001832, E5 (1)/OM001833

   
 

E5 (1)/OM001851

E2 (1)/OM001866

 
 

E1 (1)

E3 (1)/OM001867

 

E2 (2)

E1 (2)

E1 (2)

MLG-E3 (2)

E2 (1)

E1 (1)

E3 (1)

MLG-E4 (1)

E1 (1)

E6 (1)/OM001852

E4 (1)/OM001868

MLG-E5 (1)

E3 (1)

E1 (1)

  
 

E7 (1)/OM001853

E3 (1)

 

E3 (1)

E1 (1)

E1 (1)

MLG-E6 (1)

E6 (1)/OM001834

E1 (1)

E3 (1)

MLG-E7 (1)

E7 (1)/OM001835

E1 (1)

E3 (1)

MLG-E8 (1)

Sheep (Ovis aries)

63/117 (53.85)

A (2)

  

AI (1)/OM001856

 

AI (1)/OM001822

AI-1 (1)/OM001840

AI (1)

MLG-AI (1)

E (61)

 

E6 (1)

  

E8 (1)/OM001836

E7 (1)

E5 (1)/OM001869

MLG-E9 (1)

E8 (3)

E1 (3)

E6 (3)/OM001870

MLG-E10 (3)

E3 (1)

E6 (1)

E7 (1)/OM001871

MLG-E11 (1)

E9 (1)/OM001837

E1 (1)

E4 (1)

MLG-E12 (1)

E8 (1)

E6 (1)

E7 (1)

MLG-E13 (1)

E3 (1)

E1 (1)

E6 (1)

MLG-E14 (1)

  

E8 (1)/OM001872, E9 (1), E10 (1)/OM001874, E11 (1)/OM001875, E13 (1), E14 (1)/OM001878

 

E3 (2)

E6 (2)

  

E8 (1)

E1 (1)

  

E8 (1)

E7 (1)

  
 

E6 (1)

E7 (1)

 

E8 (1)

E8 (1)/OM001854

E6 (1)

MLG-E15 (1)

E3 (1)

E1 (1)

  

E10 (1)/OM001838

E8 (1)

E3 (1)

MLG-E16 (1)

E3 (2)

E1 (2)

E12 (2)/OM001876

MLG-E17 (2)

E10 (1)

 

E3 (1)

 
 

E8 (1), E9 (1)/OM001855

  
 

E8 (1)

E3 (1)

 

E3 (1)

E8 (1)

  

E8 (1)

E1 (1)

E4 (1)

MLG-E18 (1)

E8 (1)

 

E13 (1)/OM001877

 

E8 (1)

E1 (1)

E13 (1)

MLG-E19 (1)

E8 (1)

E2 (1)

E9 (1)/OM001873

MLG-E20 (1)

E2 (1)

E6 (1)

E4 (1)

MLG-E21 (1)

E3 (1)

E1 (1)

E3 (1)

MLG-E22 (1)

AI (1)

AI-1/E6 (1)

AI (1)

 

E8 (1)

AI-1 (1)

  

E8 (1)

E6 (1)

E4 (1)

MLG-E23 (1)

E3 (1)

 

E4 (1)

 

Pigs (Sus scrofa domesticus)

1/59 (1.69)

E (1)

    

Reindeer (Rangifer tarandus)

9/124 (7.26)

A (7)

AI (1)

   
 

AI-2 (2)/OM001841

  

E (2)

    

Red deer (Cervus elaphus)

7/83 (8.43)

A (1), E (6)

    

Sika deer (Cervus nippon)

5/63 (7.94)

E (5)

 

AI-1 (1)

  

Dogs (Canis lupus familiaris)

4/105 (3.81)

C (4)

  

D2 (1)/OM001864

 

Cats (Felis catus)

1/17 (5.88)

F (1)

    

Raccoon dogs (Nyctereutes procyonoides)

1/16 (6.25)

C (1)

    

Rabbits (Oryctolagus cuniculus)

67/212 (31.60)

B (67)

B2 (3)/OM001825

   
 

B2 (6)/OM001844

  
 

B2 (3)

B3 (3)/OM001860

 
 

B2 (2)

B4 (2)/OM001861

 

B2 (1)

 

B3 (1)

 

B2 (3)

B2 (3)

B4 (3)

MLG-B2 (3)

B2 (7)

B2 (7)

B3 (7)

MLG-B3 (7)

B2 (2)

B3 (2)/OM001845

B3 (2)

MLG-B4 (2)

B3 (1)/OM001826

B3 (1)

B4 (1)

MLG-B5 (1)

B2 (3)

B3 (3)

B4 (3)

MLG-B6 (3)

B2 (1)

B4 (1)/OM001846

B3 (1)

MLG-B7 (1)

B2 (1)

B2 (1)

  

B2 (1)

B3 (1)

  

B4 (1)/OM001827

B2 (1)

B4 (1)

MLG-B8 (1)

B2 (1)

 

B5 (1)/OM001862

 

Brown rats (Rattus norvegicus)

9/191 (4.71)

G (9)

G (1)/OM001839

   

G (1)

 

G (1)/OM001879

 
  

G (2)

 

Total

214/1326b (16.14)

A (10), B (67), C (5), D (1), E (121), F (1), G (9)

AI (3), B2–B4 (25), D (1), E1–E10 (42), G (2)

AI (5), B2–B4 (32), E1–E9 (49), AI + E1/E6 (2)

AI (3), B3–B5 (25), D1 (1), D2 (1), E1–E14 (41), G (3)

 
  1. The novel sequences obtained in the present study are highlighted in bold
  2. aSika deer and brown rats are wild animals, and the others are domestic animals
  3. bAbsence of G. duodenalis in goats (Capra hircus) (n = 7), horses (Equus caballus) (n = 18), arctic foxes (Vulpes lagopus) (n = 82), blue foxes (Alopex lagopus) (n = 70) and minks (Neovison vison) (n = 24) in the present study
  4. cAt the tpi locus, 19 G. duodenalis-positive specimens (in italics) were successfully sequenced using general primers and assemblage-specific primers, while the others were only sequenced successfully using assemblage-specific primers