Table 1 Data of sample collection and results of molecular analyses of carnivores and on-host ticks
Order | Family | Host species | Year of collection | Region in Hungary | PCR status of hosts for Hepatozoon/piroplasms | Result of sequencing | 18S gene GenBank accession numbers | Tick species (stage or sex) collected from hosts | Tick sp. stage (n = PCR positive for Hepatozoon [piroplasms]/all) |
|---|---|---|---|---|---|---|---|---|---|
Carnivora | Felidae | European wild cat (Felis silvestris silvestris) | 2015 | Northeast | +/− | Hepatozoon felis | OL960187 | I. kaiseri (L) | None |
2016 | Northeast | −/− | – | – | I. kaiseri (2L) | None | |||
2019 | Northeast | +/+ | Hepatozoon felis/Cytauxzoon europaeus | OM422755/OL409181 | I. ricinus (2F) | I. ricinus F: 2/2 (1/2) | |||
2021 | Northeast | +/− | Hepatozoon felis | OL960187 | – | – | |||
Mustelidae | Beech marten (Martes foina) | 2015 | Northeast | −/− | – | – | – | – | |
2017 | Northeast | +/− | Hepatozoon sp. | OL960188 | – | – | |||
2021 | North | +/− | Hepatozoon martis | OL960183 | – | – | |||
European pine marten (Martes martes) | 2017 | Northeast | +/− | Hepatozoon martis | OL960183 | I. ricinus (M, 5F, L) | I. ricinus M: 1/1, F: 5/5, L: 1/1 | ||
2019 | Northeast | +/− | Hepatozoon sp. | OL960184 | I. ricinus (2N), H. concinna (N, 8L), D. marinatus (6L), D. reticulatus (L) | I. ricinus N: 1/2; H. concinna N: 0/1, L: 3/8; D. marginatus L: 3/6; D. reticulatus L: 1/1 | |||
2019 | Northeast | +/− | Hepatozoon martis | OL960183 | H. concinna (2L) | None | |||
2020 | South | +/− | Hepatozoon martis | OL960183 | I. ricinus (F) | None | |||
Least weasel (Mustela nivalis) | 2019 | Northeast | +/− | Hepatozoon sp. | OL960185 | I. acuminatus (3F), H concinna (N, 4L) | I. acuminatus F: 1/3; H. concinna N: 0/1, L: 1/4 | ||
2015 | Northeast | −/− | – | – | – | – | |||
Wolverine (Gulo gulo) | 2020 | North (zoo) | −/− | – | – | I. hexagonus (F) | None | ||
Eurasian otter (Lutra lutra) | 2021 | Northeast | −/− | – | – | I. canisuga (3L) | None | ||
Canidae | Raccoon dog (Nyctereutes procyonoides) | 2019 | Northeast | −/− | – | – | I. ricinus (5N, 7L), H. concinna (5N, 6L) | None | |
Rodentia | Sciuridae | Red squirrel (Sciurus vulgaris) | 2017 | Northeast | −/− | – | – | I. ricinus (3N, 3L), D. marginatus (2N) | None |
2017 | Northeast | −/− | – | – | I. ricinus (2F, 8N, 3L) | None | |||
2018 | Northeast | +/− | Hepatozoon sciuri | OL960186 | – | – | |||
2019 | Northeast | +/− | Hepatozoon sciuri | OL960186 | – | – |