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Table 1 Summary details of the statistics and efficiency parameters of the three real-time PCR primer sets

From: Molecular survey of Babesia parasites in Kenya: first detailed report on occurrence of Babesia bovis in cattle

Sample name Serial dilution level DNA concentration Gene CT Y-intercept Slope Efficiency
Bbov1 10−1 14.4 ng B. bovis 18S 23.3 20.3 − 3.34 98.96
Bbov2 10−2 1.44 ng B. bovis 18S 26.9 20.3 − 3.34 98.96
Bbov3 10−3 144 pg B. bovis 18S 30.7 20.3 − 3.34 98.96
Bbov4 10−4 14.4 pg B. bovis 18S 34.2 20.3 − 3.34 98.96
Bbov5 10−5 1.44 pg B. bovis 18S 37.4 20.3 − 3.34 98.96
Bbov6 10−6 144 fg B. bovis 18S 38.3 20.3 − 3.34 98.96
Bbov7 10−7 14.4 fg B. bovis 18S 44.7 20.3 − 3.34 98.96
Bbov8 10−8 1.44 fg B. bovis 18S Undetermined 20.3 − 3.34 98.96
Bbov1 10−1 14.4 ng B. bovis Cytochrome b 17.7 14 − 3.57 90.46
Bbov2 10−2 1.44 ng B. bovis Cytochrome b 21.1 14 − 3.57 90.46
Bbov3 10−3 144 pg B. bovis Cytochrome b 24.7 14 − 3.57 90.46
Bbov4 10−4 14.4 pg B. bovis Cytochrome b 28.2 14 − 3.57 90.46
Bbov5 10−5 1.44 pg B. bovis Cytochrome b 31.7 14 − 3.57 90.46
Bbov6 10−6 144 fg B. bovis Cytochrome b 35. 7 14 − 3.57 90.46
Bbov7 10−7 14.4 fg B. bovis Cytochrome b Undetermined 14 − 3.57 90.46
Bbov8 10−8 1.44 fg B. bovis Cytochrome b Undetermined 14 − 3.57 90.46
Bbig1 10−1 51 ng B. bigemina Cytochrome b 22.3 18.9 − 3.33 99.51
Bbig2 10−2 5.1 ng B. bigemina Cytochrome b 25.7 18.9 − 3.33 99.51
Bbig3 10−3 510 pg B. bigemina Cytochrome b 28.9 18.9 − 3.33 99.51
Bbig4 10−4 51 pg B. bigemina Cytochrome b 32.4 18.9 − 3.33 99.51
Bbig5 10−5 5.1 pg B. bigemina Cytochrome b Undetermined 18.9 − 3.33 99.51
Bbig6 10−6 510 fg B. bigemina Cytochrome b Undetermined 18.9 − 3.33 99.51
Bbig7 10−7 51 fg B. bigemina Cytochrome b Undetermined 18.9 − 3.33 99.51
Bbig8 10−8 5.1 fg B. bigemina Cytochrome b Undetermined 18.9 − 3.33 99.51