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Table 1 Summary details of the statistics and efficiency parameters of the three real-time PCR primer sets

From: Molecular survey of Babesia parasites in Kenya: first detailed report on occurrence of Babesia bovis in cattle

Sample name

Serial dilution level

DNA concentration

Gene

CT

Y-intercept

Slope

Efficiency

Bbov1

10−1

14.4 ng

B. bovis 18S

23.3

20.3

− 3.34

98.96

Bbov2

10−2

1.44 ng

B. bovis 18S

26.9

20.3

− 3.34

98.96

Bbov3

10−3

144 pg

B. bovis 18S

30.7

20.3

− 3.34

98.96

Bbov4

10−4

14.4 pg

B. bovis 18S

34.2

20.3

− 3.34

98.96

Bbov5

10−5

1.44 pg

B. bovis 18S

37.4

20.3

− 3.34

98.96

Bbov6

10−6

144 fg

B. bovis 18S

38.3

20.3

− 3.34

98.96

Bbov7

10−7

14.4 fg

B. bovis 18S

44.7

20.3

− 3.34

98.96

Bbov8

10−8

1.44 fg

B. bovis 18S

Undetermined

20.3

− 3.34

98.96

Bbov1

10−1

14.4 ng

B. bovis Cytochrome b

17.7

14

− 3.57

90.46

Bbov2

10−2

1.44 ng

B. bovis Cytochrome b

21.1

14

− 3.57

90.46

Bbov3

10−3

144 pg

B. bovis Cytochrome b

24.7

14

− 3.57

90.46

Bbov4

10−4

14.4 pg

B. bovis Cytochrome b

28.2

14

− 3.57

90.46

Bbov5

10−5

1.44 pg

B. bovis Cytochrome b

31.7

14

− 3.57

90.46

Bbov6

10−6

144 fg

B. bovis Cytochrome b

35. 7

14

− 3.57

90.46

Bbov7

10−7

14.4 fg

B. bovis Cytochrome b

Undetermined

14

− 3.57

90.46

Bbov8

10−8

1.44 fg

B. bovis Cytochrome b

Undetermined

14

− 3.57

90.46

Bbig1

10−1

51 ng

B. bigemina Cytochrome b

22.3

18.9

− 3.33

99.51

Bbig2

10−2

5.1 ng

B. bigemina Cytochrome b

25.7

18.9

− 3.33

99.51

Bbig3

10−3

510 pg

B. bigemina Cytochrome b

28.9

18.9

− 3.33

99.51

Bbig4

10−4

51 pg

B. bigemina Cytochrome b

32.4

18.9

− 3.33

99.51

Bbig5

10−5

5.1 pg

B. bigemina Cytochrome b

Undetermined

18.9

− 3.33

99.51

Bbig6

10−6

510 fg

B. bigemina Cytochrome b

Undetermined

18.9

− 3.33

99.51

Bbig7

10−7

51 fg

B. bigemina Cytochrome b

Undetermined

18.9

− 3.33

99.51

Bbig8

10−8

5.1 fg

B. bigemina Cytochrome b

Undetermined

18.9

− 3.33

99.51