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Fig. 4 | Parasites & Vectors

Fig. 4

From: The mucin-like, secretory type-I transmembrane glycoprotein GP900 in the apicomplexan Cryptosporidium parvum is cleaved in the secretory pathway and likely plays a lubrication role

Fig. 4

IFA detection of GP900 protein (green) in ruptured oocysts (a, c) and excysted sporozoites (b, d) using affinity-purified polyclonal anti-GP900-C (a, b) and monoclonal anti-GP900-N (c, d) antibodies. Nuclei (blue) were counterstained with DAPI. Ruptured oocysts were prepared by subjecting intact oocysts to three freeze–thaw cycles in 4% formaldehyde. Abbreviations: DAPI, 4,6-diamidino-2-phenylindole; DIC, differential interference contrast microscopy; IFA, immunofluorescence microscopy assay

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Parasites & Vectors

ISSN: 1756-3305

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