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Fig. 9 | Parasites & Vectors

Fig. 9

From: The mucin-like, secretory type-I transmembrane glycoprotein GP900 in the apicomplexan Cryptosporidium parvum is cleaved in the secretory pathway and likely plays a lubrication role

Fig. 9

Detection of secreted GP900 protein from various parasite stages by ELISA. a Relative levels of secreted GP900 from excysted sporozoites (i.e. serum-free RPMI-1640 medium after incubation with free sporozoites following the removal of excystation medium) and intracellular parasites cultured for specified times (i.e. serum-free medium at between 0–3, 3–6, 10–12 and 20–24 hpi) using antibodies against GP900-C epitope (green), GP900-N epitope (orange) and total parasite proteins (gray). b The same optical density datasets from a after normalization with those derived from antibody against total parasite proteins. Abbreviations: ELISA, Enzyme-linked immunosorbent assay; hpi, hours post-infection

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Parasites & Vectors

ISSN: 1756-3305

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