Fig. 2

Phylogenetic tree of Wolbachia 16S in Anopheles species, with transformed branches. There were two major clades separated by significant bootstrap values 72 and 81. A subclade including haplotype 2 sequences from eastern Ethiopia was found to have significant support to be separate from the rest of the superclade. No geographic or species-specific clustering was found. The outlier sequence MH596695 did not follow the clustering of Wolbachia supergroup A or B, so it was not included in the distinction between these supergroups. Rickettsia japonica was used as the outgroup (NR074459). Haplotype 1 is designated by light blue font, haplotype 2 is designated by red font, and haplotype 3 is designated by orange font