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Fig. 1 | Parasites & Vectors

Fig. 1

From: Characterization of PSOP26 as an ookinete surface antigen with improved transmission-blocking activity when fused with PSOP25

Fig. 1

PSOP26 transcript levels, protein expression, and localization on the surface of gametes and ookinetes in P. berghei. a PSOP26 RT-PCR transcription profiles. Real-time RT-PCR analysis of the expression profile of psop26 transcripts in schizonts (Sch), non-activated gametocytes (Gam), and ookinetes (Ook). The β-tubulin gene, which is constitutively expressed at all three stages, was used as a loading control. b Expression of PSOP26::HA during parasite development. Western blot analysis of proteins extracted from purified P. berghei schizonts (Sch), gametocytes (Gam), zygotes (Zyg), and ookinetes (Ook) of PSOP26-HA parasites. Reduced lysates were separated by SDS-PAGE, transferred to a PVDF membrane, and labeled with anti-HA antibody and anti-Hsp70 sera (loading control). Non-infected erythrocytes (EC) were used as a negative control. c Immunofluorescence assays of Triton X-100 permeabilized (+Triton X-100, left) or non-permeabilized (−Triton X-100, right) P. berghei ring, trophozoite, schizont, gametocytes, gametes, zygotes, and ookinetes. Stained with α-HA monoclonal antibody (green), α-msp1 (merozoite surface protein, red) and/or α-tubulin II (male gametocyte/gamete protein, red), and/or α-Pbg377 (female gametocyte/gamete protein, red), and/or α-PSOP25 (ookinete surface protein). DNA was stained with DAPI. Only the secondary antibodies or WT ookinetes incubated with the α-HA mAb were used as a negative control (AF488, Alexa Fluor 488, AF555, and Alexa Fluor 555). Merge denotes Alexa Flour 488 + Alexa Flour 555 + DAPI. Scale bars correspond to 5 μm

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