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Fig. 2 | Parasites & Vectors

Fig. 2

From: Characterization of PSOP26 as an ookinete surface antigen with improved transmission-blocking activity when fused with PSOP25

Fig. 2

Phenotypic analyses of ∆psop26 mutants in asexual and sexual stages. Mice were injected with WT or ∆psop26 parasites clone 1 (C1) and clone 2 (C2). a Survival rates of mice. b Parasitemia was monitored by Giemsa smears after day 3 infection. c Gametocytemia (mature gametocytes per 104 RBCs) on day 3 post-infection. d Gametocyte sex ratios (female: male) on day 3 post-infection with different parasite lines. Error bars indicate mean ± SEM (standard error of the mean) (n = 3). Data in the phenotypic analysis of pbs54 and psop26 are representative of three independent experiments. e Exflagellation centers in 10 fields at × 100 magnification with equal number of mature male gametocytes. f Female gamete numbers in 0.5 µl of ookinete culture at 15 min post-activation. g Zygote numbers formed at 2 h during in vitro ookinete culture. h Ookinete numbers cultured in vitro for 24 h. i Oocyst numbers per midgut in mosquitoes 12 days after feeding on WT, ∆pbs54, or ∆psop26 parasite-infected mice. **P < 0.01 (Mann–Whitney U-test). Error bars indicate mean ± SEM (n = 3). Data in the phenotypic analysis of psop26 are representative of three independent experiments

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