Skip to main content
Fig. 3 | Parasites & Vectors

Fig. 3

From: Characterization of PSOP26 as an ookinete surface antigen with improved transmission-blocking activity when fused with PSOP25

Fig. 3

Purification of recombinant cPSOP25/26 protein and antibody response in mice immunized with rPSOP25, rPSOP26, rPSOP25 + PSOP26, and PSOP25-PSOP26. a Diagram illustrating the expressed regions of the PSOP26, PSOP25, and chimeric PSOP25-PSOP26. The signal peptide (red box), low complexity (green box), and transmembrane region (blue box) are highlighted. The pink line denotes the linker. b Purified recombinant PSOP25-PSOP26 (indicated by an arrow) was separated on a 10% SDS-PAGE gel and stained with Coomassie blue (left) and probed with anti-His mAb on a Western blot (right). BALB/c mice (n = 10) were immunized three times with Trx-His tag (immunization control) and recombinant proteins (rPSOP25, rPSOP26, rPSOP25 + PSOP26, and rPSOP25-PSOP26). Total IgG titers after final immunization were measured by ELISA analysis coated with recombinant PSOP25 (c) and recombinant PSOP26 (d) polypeptides after removal of the Trx tag. Error bars indicate standard deviation. **P < 0.01 indicates a significant difference between the immunization and control groups (Student’s t-test)

Back to article page