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Fig. 5 | Parasites & Vectors

Fig. 5

From: Characterization of PSOP26 as an ookinete surface antigen with improved transmission-blocking activity when fused with PSOP25

Fig. 5

Transmission-blocking activity of the antisera assessed in vitro and in vivo. a Exflagellation centers per 10 fields. Plasmodium berghei-infected blood collected at 3 dpi was incubated with the respective control and immune sera at dilutions of 1:5 and 1:10. b Ookinete formation. The ookinete numbers after 24 h were stained with Pbs21 mAb and counted using a fluorescence microscope. The data were representative of three independent experiments. c Midgut oocyst numbers in mosquitoes fed on relative recombinant protein-immunized mice. The results were collected from three mice in each immunization group and two separate experiments (n = 180). Data points represent midgut oocyst numbers in individual mosquitoes. Horizontal bars indicate the mean number of oocysts per midgut. d Mosquito infection prevalence was calculated at 12 days after the blood meal. Data points represent the prevalence of infection in mosquitoes from three mice per group and in two separate experiments. Error bars indicate mean ± SD. *P < 0.05 and ***P < 0.001 represent the significant difference between the respective immunization group and the Trx-His control group. #P < 0.05 # represents a significant difference between two immunization groups

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