Skip to main content
Fig. 1 | Parasites & Vectors

Fig. 1

From: Depletion of Toxoplasma adenine nucleotide translocator leads to defects in mitochondrial morphology

Fig. 1

Identification and localization of TgANT protein. a Domain architecture of TgANT in Toxoplasma gondii. The domains were predicted by databases of protein families (Pfam). b Three-dimensional (3D) structures of TgANT predicted by Ab Initio Modeling using trRosetta. The quality of trRosetta modeling results is mainly based on its TM-score, which is between 0 and 1. The closer to 1, the higher the accuracy of the modeling and the greater the credibility. c Strategy for the construction of the strain with the FLAG endogenous epitope tag. d Western blot confirmed the expression of FLAG-tagged TgANT in parasites. Actin was used as a control. e IFA assays indicated that TgANT showedthe typical lasso-shaped mitochondria-like localization in intracellular tachyzoites. Parasites were labeled with rabbit anti-GAP45 (green), mouse anti-FLAG (red) and DAPI (blue). Scale bar, 2 μm. f IFA results showed the colocalization of anti-TgANT signal (green) and MitoTracker (red). MitoTracker was used as a mitochondrial marker. Scale bar: 2 μm. Abbreviations: CAT, chloramphenicol; DAPI,4′,6-Diamidino-2-phenylindole; IFA, immunofluorescence assay; TgANT, adenine nucleotide translocase in T. gondii

Back to article page