Fig. 2

Three-dimensional structure of the PvRBP2b reticulocyte binding region. a A modified schematic diagram from [14] shows the important residues in the reticulocyte binding region composed of the N-terminal and C-terminal domains. The polymorphic residues previously reported are labeled in magenta, whereas the polymorphic residues newly identified in the present study are labeled in red. Residues interacting with TfR1 and Tf are shown in green and cyan, respectively. b Two orthogonal views of the three-dimensional model structure of the PvRBP2b reticulocyte binding region shows the 14 residues positively selected by both codon-based tests in the Datamonkey webserver and S586 positively selected by FUBAR. The hydrophobic binding region is shown in yellow. Abbreviations: Tf, transferrin; TfR1 transferrin receptor 1