Fig. 7

Characteristics of the subsets of Tregs during P.y17XL infection. Triple staining with FITC-anti-CD4, PE-anti-CD25 and APC-anti-Foxp3 was used to detect the proportion (a), absolute cell numbers (b) (column diagram, left) and representative contour (right) of Tregs (c) in P.y17XL-infected BALB/c mice. Results are shown as the mean of four mice per group ± SEM. Asterisks indicate significant difference at *P < 0.05 and **P < 0.01 vs. control mice (non-infected mice, 0 days); hashtags indicate significant difference at ^#P < 0.05 and ^##P < 0.01 in mice at 3 vs. 5 days p.i, using one-way analysis of variance. Abbreviations: Tregs, T regulatory cells