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Fig. 3 | Parasites & Vectors

Fig. 3

From: Balamuthia mandrillaris trophozoites ingest human neuronal cells via a trogocytosis-independent mechanism

Fig. 3

A Schematic diagram of the differential cell tracking of CMFDA-labeled human neuroblastoma cells (green) and DiD-labeled B. mandrillaris trophozoites (magenta), followed by coculture. B Cell-to-cell interactions of the DiD-labeled trophozoites (magenta) with human SH-SY5Y cells (green for CMFDA and blue for DAPI) at 40 min post coculture. C Invadopodia of the B. mandrillaris trophozoites protruding into human neuroblastoma cells. Arrows, the cytoplasm of human cells; Yellow arrow, invadopodia-like structure.  D The X–Y-Z plane of confocal images of the DiD-labeled trophozoites (magenta) with human SH-SY5Y cells (green for CMFDA and blue for DAPI). Yellow arrow, invadopodia-like structure. E Schematic diagram of the cellular uptake of unlabeled B. mandrillaris trophozoites. F Confocal images of the B. mandrillaris trophozoites along the X–Y plane. Dotted line, the cytoplasm of the B. mandrillaris trophozoites; Arrowheads, human nuclei.  G Proportion of B. mandrillaris trophozoites that ingested human cell components (magenta circles). Abbreviations: DAPI, 4′,6-Diamidino-2-phenylindole; DiD, 1, 1-dioctadecyl-3,3,3,3-tetramethylindodicarbocyanine; PCMFA, 5-chloromethylfluorescein diacetate; PFA, paraformaldehyde

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