Fig. 1
From: Systematic identification of the lysine lactylation in the protozoan parasite Toxoplasma gondii
Overview of the Toxoplasma gondii lactylome. A WB analysis of tachyzoite lysate using lactyllysine antibody antibody demonstrate the presence of lactylated proteins in both extracellular (extra) and intracellular (intra) tachyzoites. The upper and lower panels show WB results and equal loading amounts by SDS-PAGE, respectively. B WB analysis of the regulatory effect of sodium lactate on intracellular parasites. The upper and lower panels show WB results and equal loading amounts by SDS-PAGE, respectively. C LC–MS/MS spectrum database search analysis summary. D Distribution of Kla proteins based on the number of modification sites. Abbreviations: IB, immunoblot; Kla, Lysine lactylation; LC-MS/MS, liquid chromatography-tandem mass spectrometry; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; Pan Kla, pan-lactyllysine; WB, western blotting