Impact of wAlbB Wolbachia on oviposition (a) and egg hatching rate (b) of WAG females. Box-and-whisker plot (a) represents the number of eggs laid per female in the respective Wolbachia-infected and non-infected groups. The bottom and top of the box represent the first (lower) and third (upper) quartiles (25–75th percentile), respectively, and the middle line within the box indicates the median. The cross denotes the mean. The vertical lines (ends of the whiskers) extend to the minimum and maximum values. P-values obtained by ANOVA at 95% confidence interval to compare the difference of means of the three groups are mentioned at the top of a and P-values obtained using a Chi-square test to compare the three groups are given at the top of b. Abbreviations: ANOVA, Analysis of variance; RAG, uninfected Aedes aegypti (control); TWAG, wAlbB Wolbachia removed by tetracycline; WAG, wAlbB Wolbachia-transfected colony treatment.