Fig. 1

Evolutionary relationships of 46 Trypanosoma cruzi FeSOD-A gene sequences. Nucleotide sequences obtained in this study are named according to the T. cruzi strains (see Table 1) and identifiers come from GenBank. Sequences from public databases are named according to the ENA database, except for CL_Brener.XM_807064, which comes from the RefSeq database (see Table 2). The Tulahuen gene sequence was removed because it appears to cause a tree artifact. Discrete typing units are highlighted: TcI (blue), TcII (orange), TcIII (red), TcIV (green), TcV (gray) and TcVI (pink). The alignment comprises a total of 573 sites. The phylogeny was reconstructed by two methods using the best fit model (Kimura-2 parameter) and estimation of the proportion of invariable sites. In the Bayesian inference, support values for each node were estimated as posterior probability (numbers in black above node). In the maximum likelihood analysis, they were estimated using the bootstrap method (numbers in red below node). Only support values higher > 70% are shown