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Fig. 4 | Parasites & Vectors

Fig. 4

From: Expression of fatty acid synthase genes and their role in development and arboviral infection of Aedes aegypti

Fig. 4

Expression of AaFAS in mosquito developmental stages and sexes. RNA was prepared from five specimens of fourth-instar larvae, pupae, sugar-fed males, sugar-fed females and blood-fed females (3-days pbm) (all adult mosquitoes were collected at the same day time; 8–10 days post eclosion). Samples were subjected to RT-qPCR to assess relative expression of AaFAS1-5 and AaFAS-like. RNA levels between samples were normalized to the β-actin gene using 2−ΔCt method. The boxes show the 25th and 75th percentiles, the whiskers show the minimum and maximum values. The midline indicates the median of the relative gene expression value. The experiment was repeated twice, and representative results from a single experiment are shown

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