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Fig. 7 | Parasites & Vectors

Fig. 7

From: Expression of fatty acid synthase genes and their role in development and arboviral infection of Aedes aegypti

Fig. 7

Assessment of RNAi-induced AaFAS1 knockdown on DENV2 replication in Aag2 cells. a Percent AaFAS1 expression in AaFAS1-KD Aag2 cells compared to the AaFAS1 expression in GFP-KD cells. Expression was measured at 2 days post dsRNA transfection. b RNA replication of luciferase-expressing DENV2 in Aag2 cells treated with dsRNA derived from AaFAS1, DENV2 (positive KD control) or GFP (negative KD control). Cells were transfected with dsRNA for 2 days prior to infection with luciferase-tagged DENV2. At 24 h post infection, cells were lysed and were assayed for luciferase activity (RLU). c Viability of Aag2 cells treated with dsRNA derived from AaFAS1, DENV2 and GFP genes assessed by resazurin assay. All treatment were performed on three biological replicates. The experiment was repeated twice, and representative results from a single experiment are shown

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