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Table 3 Molecular identification of Trypanosoma species with the ITS1 gene

From: Parasites and blood-meal hosts of the tsetse fly in Tanzania: a metagenomics study

No.

Sample ID

Identification of Trypanosoma sp. (accession, similarity %)

Host

Region

Sex of the tsetse fly

1

M10

–*

Human

African buffalo

Maswa Game Reserve

Female

2

M21

Trypanosoma simiae (JN673382, 100%)

Human

African elephant

Maswa Game Reserve

Female

3

M60

PCR failed

Human

Common warthog

Mouse

Maswa Game Reserve

Male

4

S17

Trypanosoma godfreyi

(JN673383, 95.3%)

NGS failed

Serengeti National Park

Female

5

S55

Trypanosoma godfreyi

(MK131836, 99.4%)

African buffalo

Serengeti National Park

Male

6

T73

PCR failed

Human

Mouse

Tarangire National Park

Male

  1. PCR was performed with primers targeting the Trypanosoma-specific ITS1 gene, and conventional DNA sequencing was performed to identify Trypanosoma species
  2. *DNA sequencing failed because of weak amplicon band