Screening and phylogenetic characterization of tick-borne pathogens in a population of dogs and associated ticks in Egypt

Table 2 Thermal profile used for PCR procedures

Parasite Step	Babesia		Cycle no.	Hepatozoon canis		Cycle NO	Anaplasmataceae		Cycle no.
Parasite Step	Temp	Time	Cycle no.	Temp	Time	Cycle NO	Temp	Time	Cycle no.
Initial denaturation	94 °C	5 min	1	95 °C	5 min	1	95 °C	5 min	1
Denaturation	94 °C	30 Sec	35	95 °C	30 Sec	35	95 °C	30 Sec	40
Annealing	49 °C	30 Sec		51 °C	30 Sec		55 °C	30 Sec
Extension	72 °C	1 min		72 °C	1.5 min		72 °C	30 Sec
Final extension	72 °C	10 min	1	72 °C	10 min	1	72 °C	10 min	1

ISSN: 1756-3305