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Fig. 1 | Parasites & Vectors

Fig. 1

From: Pyrokinin receptor silencing in females of the southern cattle tick Rhipicephalus (Boophilus) microplus is associated with a reproductive fitness cost

Fig. 1

Rhimi-PKR relative transcript expression at different stages of development, measured by qRT-PCR. Stages of development analyzed were as follows using 8 biological replicates of each of the following stages: egg masses (Egg), neolarvae (Larva), nymph, male and female. qRT-PCR results showed that the Rhimi-PKR transcript was expressed throughout all stages of development, with the lowest expression observed for eggs and males. The relative Rhimi-PKR abundance for eggs was used as the reference ratio (calibrator) for the FC calculations. Rhimi-PKR abundance was similar for neolarva (Larva in histogram), nymph and female, and higher than in egg by FC factors of 4.3, 4.7 and 4.3 (n = 8 biological replicates; P < 0.05), respectively. Rhimi-PKR abundance for male was the second lowest, and not different from that in egg (n = 8; P > 0.05). A one-way ANOVA followed by a Tukey’s multiple comparisons test was used for the statistical analysis. Different lowercase letters at top of histogram bars indicate significant difference. Abbreviations: ANOVA, Analysis of variance; FC, fold change; qRT-PCR, quantitative reverse-transcriptase PCR; Rhimi-PKR, Rhipicephalus microplus-pyrokinin receptor

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