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Fig. 3 | Parasites & Vectors

Fig. 3

From: Pyrokinin receptor silencing in females of the southern cattle tick Rhipicephalus (Boophilus) microplus is associated with a reproductive fitness cost

Fig. 3

Phenotypic evaluation of Rhimi-PKR silencing in Rhipicephalus microplus females. Results for both treatments, PKR ds762-913 and ds1485-1627 were compiled for the analysis of all the phenotypic variables: a percentage of female mortality, b duration of repletion period, c female weight, d duration of pre-oviposition period, e egg mass weight, f REI, which represents the conversion of the blood intake to egg mass production, g females with no eggs, i.e. females that showed not oviposition at all, h duration of egg incubation period, i percentage of egg hatching per female, j females with no hatch, i.e. percentage of females whose egg mass had no eggs hatching at all, k total observation period, which represents the time period from “tick attachment to animal” until hatching of the first egg for each female. Positive control: Rhimi-ACTB dsRNA-injected ticks (dsβ-act). Treatments sharing the same lowercase letter (top of each graph) were not significantly different from each other (P < 0.05) as determined by the Kruskal–Wallis test followed by the corrected Dunn’s multiple comparisons test. Abbreviations: NI, Negative controls (non-injected ticks); dsβ-lac, beta-lactamase dsRNA-injected ticks; dsPKR, Rhimi-PKR dsRNA-injected ticks; REI, reproductive efficiency index

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