Pyrokinin receptor silencing in females of the southern cattle tick Rhipicephalus (Boophilus) microplus is associated with a reproductive fitness cost

Wulff, Juan P.; Temeyer, Kevin B.; Tidwell, Jason P.; Schlechte, Kristie G.; Xiong, Caixing; Lohmeyer, Kimberly H.; Pietrantonio, Patricia V.

doi:10.1186/s13071-022-05349-w

Table 1 Oligonucleotide primers for cloning, double-stranded RNA synthesis and quantitative reverse-transcriptase PCR

From: Pyrokinin receptor silencing in females of the southern cattle tick Rhipicephalus (Boophilus) microplus is associated with a reproductive fitness cost

Primer name	Oligo sequence 5ʹ-3′^a	Notes
RmPyro93U27	TGGAACTGTCAAGCAGGCTGAGGCAGA	Dualluc construction
RmPYro1625L30	AGAGAGTAAGCTTTCGCAGGCAAAATACAC	Dualluc construction
DualLucRmPyr-8U18	CGTCAGCGGGCTTCGAACAAGCAGGCTGAGGCAGA	Dualluc construction (insert)
DualLucPyr-1538L21	GAATACTGTGGGAGCTCGCTA	Dualluc construction (insert)
DualLucPyro1789U21	GAATACTGTGGGAGCTCGCTA	Dualluc construction (vector)
DualLucPyro8198L16	TTCGAAGCCCGCTGAC	Dualluc construction (vector)
RmPyro-762U20	GCGAGAGGAGCCTCAACGAG	Rhimi-PKR dsRNA synthesis
RmPyro-913L20	GTACCGGTGTCGTCTTCGTC	Rhimi-PKR dsRNA synthesis
RmPyro-762U20T7	AAAGGCCTTAATACGACTCACTATAGGGGCGAGAGGAGCCTCAACGAG	Rhimi-PKR dsRNA synthesis
RmPyro-913L20T7	AAAGGCCTTAATACGACTCACTATAGGGTCGGTGTCGTCTTCGTC	Rhimi-PKR dsRNA synthesis
RmPyro-1485U16	TCGCCGCCAAGTACAG	Rhimi-PKR dsRNA synthesis
RmPyro-1627L27	GAGAGTAAGCTTTCGCAGGCAAAATAC	Rhimi-PKR dsRNA synthesis
RmPyro-1485U16-T7	AAAGGCCTTAATACGACTCACTATAGGGTCGCCGCCAAGTACAG	Rhimi-PKR dsRNA synthesis
RmPyro-1627L27-T7	AAAGGCCTTAATACGACTCACTATAGGGAGAGTAAGCTTTCCAGGCAAAATAC	Rhimi-PKR dsRNA synthesis
Amp-fwd	CGCTGGTGAAAGTAAAATATG	Beta-lactamase dsRNA synthesis [28]
Amp-rev	GCCGGGAAGCTAGAGTAAGTA	Beta-lactamase dsRNA synthesis [28]
Amp-T7	TAATACGACTCACTATAGGGCGCTGGTGAAAGTAAAATATG	Beta-lactamase dsRNA synthesis [28]
Amp-T7	TAATACGACTCACTATAGGGCCGGGAAGCTAGAGTAAGTA	Beta-lactamase dsRNA synthesis [28]
BmBActin-1U20	TCCTCGTCCCTGGAGAAGTC	Rhimi-ACTB dsRNA synthesis [28]
BmBActin-285L18	GGGGGAGCGATGATCTTG	Rhimi-ACTB dsRNA synthesis [28]
BmbActin-1U20-T7	TAATACGACTCACTATAGGGTCCTCGTCCCTGGAAGAAGTC	Rhimi-ACTB dsRNA synthesis [28]
BmbActin-285L18-T7	TAATACGACTCACTATAGGGGGAGCGATGATCTTG	Rhimi-ACTB dsRNA synthesis [28]
RmPKR-qF2	ACGCGCCATGAATGGAA	Rhimi-PKR qRT-PCR [23]
RmPKR-qR2	GTGTGAAGCTGGTGGTTTGAGA	Rhimi-PKR qRT-PCR [23]
BmbA-1528-F	CAAACGGAGGTGGAGCTGTC	Rhimi-ACTB qRT-PCR [28]
BmbA-1629-R	GCTAGAATATGTGAGGGCGCGAC	Rhimi-ACTB qRT-PCR [28]
BmELF1a-88-F	CGTCTACAAGATTGGTGGCATT	Rhimi-EF1A qRT-PCR [39]
BmELF1a-196-R	CTCAGTGGTCAGGTTGGCAG	Rhimi-EF1A qRT-PCR [39]
RmRPS4-qF1	TCATCCTGCACCGCATCA	Rhimi-RPS4 qRT-PCR [23]
mRPS4-qR1	ACGCGGCACAGCTTGTACT	Rhimi-RPS4 qRT-PCR [23]

ACTB Beta-actin gene, ds double-stranded, EF1A elongation factor 1 alpha gene, PKR pyrokinin receptor, qRT-PCR quantitative reverse-transcriptase PCR, Rhimi Rhipicephalus microplus, genus and species, RPS4 ribosomal protein S4 gene
^aSequences in italics were added to the primers and are not part of the tick complementary DNA sequences

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ISSN: 1756-3305

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