Table 2 Positivity rate by fresh examination, differential count and kinetoplast DNA–PCR analysis in excreta and intestinal contents of R. robustus and R. pictipes fed on Swiss mice previously infected with 1400 blood trypomastigotes of different Trypanosoma cruzi strains as pure or mixed inocula
Experimental group (n = 20) | Rhodnius robustus | Rhodnius pictipes | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
% of positivity of excretaa | % of positivity of ICa | % of positivity of excretaa | % of positivity of ICa | |||||||||
FE | DC | kDNA-PCR | FE | DC | kDNA-PCR | FE | DC | kDNA-PCR | FE | DC | kDNA-PCR | |
G1 (TcIAM) | 0.0 | 0.0 | 100.0 | 0.0 | 12.5 | 75.0 | 10.0 | 30.0 | 70.0 | 37.5 | 50.0 | 75.0 |
G2 (TcIMG) | 12.5 | 0.0 | 90.0 | 12.5 | 12.5 | 50.0 | 0.0 | 0.0 | 90.0 | 12.5 | 50.0 | 87.5 |
G3 (TcII) | 10.0 | 0.0 | 100.0 | 25.0 | 25.0 | 87.5 | 10.0 | 0.0 | 70.0 | 75.0 | 87.5 | 100.0 |
G4 (TcIV) | 30.0 | 20.0 | 90.0 | 25.0 | 37.5 | 87.5 | 0.0 | 10.0 | 70.0 | 37.5 | 75.0 | 75.0 |
G5(TcIAM+TcIV) | 10.0 | 30.0 | 100.0 | 25.0 | 75.0 | 87.5 | 0.0 | 10.0 | 100.0 | 0.0 | 0.0 | 100.0 |
G6 (TcIAM+TcII) | 0.0 | 0.0 | 100.0 | 0.0 | 0.0 | 87.5 | 20.0 | 10.0 | 100.0 | 37.5 | 37.5 | 100.0 |
G7 (TcIMG+TcIV) | 10.0 | 0.0 | 80.0 | 25.0 | 50.0 | 62.5 | 0.0 | 0.0 | 60.0 | 25.0 | 25.0 | 100.0 |
G8 (TcIMG+TcII) | 10.0 | 0.0 | 100.0 | 37.5 | 37.5 | 75.0 | 0.0 | 0.0 | 100.0 | 25.0 | 12.5 | 37.5 |
G9 (TcII+TcIV) | 10.0 | 0.0 | 100.0 | 12.5 | 12.5 | 75.0 | 0.0 | 0.0 | 80.0 | 12.5 | 25.0 | 37.5 |
P value* | 0.0116 |  < 0.0001 | 0.001 | ns | ns | 0.039 | 0.001 | 0.002 | ns | ns | ns | 0.012 |