Table 1 Primers and probes

Tick-borne encephalitis virus

F-TBE1

5′-GGGCGGTTCTTGTTCTCC-3′

Primers, 5′ untranslated region

[28]

R-TBE1

5′-ACACATCACCTCCTTGTCAGACT-3′

TBE-WT

5′-FAM-TGAGCCACCATCACCCAGACACA-RTQ1-3′

Probe, 5′ untranslated region

Borrelia sp.

BspF-16s

5′-GCTGTAAACGATGCACACTTGGT-3′

Primers, 16S rRNA gene of B. sp.

[29, 30]

BspR-16s

5′-GGCGGCACACTTAACACGTTAG-3′

FAM-LD

5′-FAM-TTCGGTACTAACTTTTAGTTAA-MGB-3′

Probe, 16S rRNA gene of B. burgdorferi s.l

VIC-RF

5′-VIC-CGGTACTAACCTTTCGATTA-MGB-3′

Probe, 16S rRNA gene of B. miyamotoi

Bfla-PAD

5′-GATCARGCWCAAYATAACCAWATGCA-3’

Primers, flagellin B gene of Borrelia sp.

[31]

Bfla-PDU

5′-AGATTCAAGTCTGTTTTGGAAAGC-3’