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Table 2 MALDI-TOF MS identification results of species from different origins after crosswise testing against DB2 and DB3

From: Enhanced procedures for mosquito identification by MALDI-TOF MS

Query against databasesa

Species

Originb

Numberc

Correct species, n (%)d

Incorrect species, n (%)d

Heads

Thoraxes

Legs

Heads

Thoraxes

Legs

BD2

Ae. aegyptie

French Polynesia (Bora)

15

15 (100)

15 (100)

15 (100)

0 (0.0)

0 (0.0)

0 (0.0)

Ae. aegypti

Brazil (Oia)

15

15 (100)

15 (100)

15 (100)

0 (0.0)

0 (0.0)

0 (0.0)

Ae. albopictuse

France (Mrs)

13

13 (100)

13 (100)

13 (100)

0 (0.0)

0 (0.0)

0 (0.0)

Ae. albopictus

Cameroon (Dsg)

15

15 (100)

15 (100)

13 (86.7)

0 (0.0)

0 (0.0)

2 (13.3)

An. coluzzie

Senegal (Dkr)

15

5 (33.3)

12 (80.0)

15 (100)

10 (66.7)

3 (20.0)

0 (0.0)

An. gambiae s.s.e

Kenya (Kis)

18

18 (100)

18 (100)

18 (100)

0 (0.0)

0 (0.0)

0 (0.0)

An. coluzzii

Cameroon (Tib)

16

4 (25.0)

0 (0.0)

4 (25.0)

12 (75.0)

16 (100)

12 (75.0)

An. coluzzii

Congo (Bra)

3

2 (66.7)

0 (0.0)

0 (0.0)

1 (33.3)

3 (100)

3 (100)

An. gambiae s.s.e

Congo (Bra)

14

11 (78.6)

14 (100)

11 (78.6)

3 (21.4)

0 (0)

3 (21.4)

Total (%)

  

124

98 (79.0)

102 (82.3)

104 (83.9)

26 (21.0)

22 (17.7)

20 (14.5)

BD3

Ae. aegyptie

French Polynesia (Bora)

15

15 (100)

15 (100)

15 (100)

0 (0.0)

0 (0.0)

0 (0.0)

Ae. aegypti

Brazil (Oia)

13

13 (100)

13 (100)

13 (100)

0 (0.0)

0 (0.0)

0 (0.0)

Ae. albopictuse

France (Mrs)

13

13 (100)

13 (100)

13 (100)

0 (0.0)

0 (0.0)

0 (0.0)

Ae. albopictus

Cameroon (Dsg)

13

13 (100)

13 (100)

13 (100)

0 (0.0)

0 (0.0)

0 (0.0)

An. coluzzie

Senegal (Dkr)

15

5 (33.3)

13 (86.7)

15 (100)

10 (66.7)

2 (13.3)

0 (0.0)

An. gambiae s.s.e

Kenya (Kis)

18

18 (100)

18 (100)

18 (100)

0 (0.0)

0 (0.0)

0 (0.0)

An. coluzzii

Cameroon (Tib)

14

10 (71.4)

6 (42.9)

5 (35.7)

4 (28.6)

8 (57.1)

9 (64.3)

An. coluzzii

Congo (Bra)

3

2 (66.7)

0 (0.0)

1 (33.3)

1 (33.3)

3 (100)

2 (66.7)

An. gambiae s.s.e

Congo (Bra)

12

4 (25.0)

11 (91.7)

5 (41.66)

8 (75.0)

1 (8.3)

7 (58.3)

Total (%)

  

116

93 (80.2)

102 (87.9)

98 (84.5)

23 (19.8)

14 (12.1)

18 (15.5)

  1. s.s. sensu stricto
  2. aSee Table 1 for details on DB2 and DB3
  3. bCountry of origin of the sample (abbreviation for the name of each strain is given in parentheses)
  4. cNumber of samples blindly tested against databases (DB2 or DB3)
  5. dn: number of samples with correct (same name as determined by molecular methods) or incorrect (different name from that determined with molecular methods) species identification. %: prevalence or proportion of samples
  6. eMosquito strains were laboratory reared
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