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Fig. 5 | Parasites & Vectors

Fig. 5

From: Eimeria falciformis secretes extracellular vesicles to modulate proinflammatory response during interaction with mouse intestinal epithelial cells

Fig. 5

Eimeria falciformis sporozoite EVs modified mRNA expressions of IL-6, IL-17, MCP1, IL-1β and IL-18. MIECs (1 × 106) were plated and stimulated with 50 µg of EfSEVs for 6, 12 and 18 h and with 10, 30, 50 and 100 µg of EfSEVs for 24 h. PBS (0 µg of EfSEVs) was used as an experimental control for dose treatments and 0 h was used as control for time-dependent treatments. An equal volume of NC particles was set as negative control. After each time point, RNAs were extracted, and IL-6, IL-17, MCP1, IL-1β and IL-18 expressions were determined by quantitative PCR. Bars represent mean ± standard deviation values in triplicate experiments. Asterisks indicate a statistical significant difference with the experimental control treatment at *P < 0.05, **P < 0.001, ***P < 0.001

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