Genomic sequence capture of Plasmodium relictum in experimentally infected birds

Parasites & Vectors

Table 1 BLAST results of unmapped reads against the refseq database for five samples representing the three lineages in this study (GRW11, GRW4, SGS1) and the two SGS1 lineage isolates (SGS1-A and SGS1-B)

BLAST Taxon Hit	GRW11 (cc82)	GRW4 (51242)	SGS1-A (1309)	SGS1-A (1455)	SGS1-B (735)
Aves: Passeriformes	6342	39,851	2258	14,009	1900
Aves: Non-passerine orders	322	3734	89	910	90
Mammalia	10	150	3	31	4
Plasmodium	0	0	0	0	0
All hits	16,478	82,689	9782	30,536	5467
Total unmapped reads	991,173	14,761,081	360,028	1,593,536	423,348
No BLAST hit	974,695	14,678,392	350,246	1,563,000	417,881
All reads	6,116,400	15,219,066	5,915,344	1,839,486	1,939,288

Two samples from the SGS1-A lineage isolate were BLASTed; they represent a high-parasitemia (1309) and low-parasitemia (1455) sample. The number of reads that mapped to birds (shown separately for Passeriformes and non-passerine orders), mammals (Mammalia), and Plasmodium (no unmapped reads mapped to Plasmodium) are shown. The “All hits” category is the sum of the reads in the aforementioned categories and all other unmapped reads that resulted in hits to other taxonomic categories (including unassigned taxa; data not shown). The number of unmapped reads that did not result in a BLAST hit (“No BLAST hit”), the total number of unmapped reads for each sample (“Total unmapped reads”), and the total number of sequenced reads for each sample (mapped and unmapped; “All reads”) are also shown. Sample name follows lineage name in parentheses

ISSN: 1756-3305