Fig. 2

Differential expression of lncRNA transcripts and mRNAs in the spleen of Beagle puppies infected by Toxocara canis at 24 hpi, 96 hpi, and 36 dpi. a Number of DElncRNAs transcripts and DEmRNAs at three infection stages. Blue, red and green colors represent total, upregulated or downregulated total transcripts and genes, respectively. b–d Verification of the expression of DElncRNAs and DEmRNAs using qRT-PCR at 24 hpi (b), 96 hpi (c) and 36 dpi (c). Y-axis denotes the log2 fold change, and X-axis shows the analyzed DElncRNAs and DEmRNAs. Error bars represent the standard deviation based on three replicates. DElncRNAs, Differentially expressed lncRNA transcripts; DEmRNAs, differentially expressed mRNAs; dpi, days post-infection; hpi, hours post-infection; qRT-PCR, quantitative real-time PCR; RNA-seq, RNA sequencing