Concurrent molecular characterization of sand flies and Leishmania parasites by amplicon-based next-generation sequencing

Table 1 Primer sequences and corresponding target genes used in the study

Primer name	DNA sequences	Target gene /PCR product size	References
ITS1219NGSF	TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGAGCTGGATCATTTTCCGATG	Leishmania ITS1/343 bp	[33]
ITS1219NGSR	GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGATCGCGACACGTTATGTGAG	Leishmania ITS1/343 bp	[33]
SFNGSF	TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGTGCGGTTAAAACGTTCGTAG	Sand flies 18S rDNA/230 bp	[44]
SFNGSR	GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGACCGGTAAAACATCCGTCAC	Sand flies 18S rDNA/230 bp	This study

ISSN: 1756-3305