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Fig. 2 | Parasites & Vectors

Fig. 2

From: Toxoplasma gondii dense granule protein 3 promotes endoplasmic reticulum stress-induced apoptosis by activating the PERK pathway

Fig. 2

Expression of dense granule protein 3 (GRA3Wh6). Mouse neuroblastoma (N2a) cells were transfected with either a control vector (pEGFP, plasmid encoding enhanced green fluorescent protein) or pEGFP-GRA3Wh6 fusion protein (GRA3Wh6) for a period of 24 h. Mock-transfected N2a cells served as a negative control. a The expression of green fluorescent protein (GFP) in pEGFP and pEGFP-GRA3Wh6 transfected N2a cells was observed using fluorescence microscopy. Scale-bar 20 µM. b The GFP intensity in pEGFP and pEGFP-GRA3Wh6-transfected N2a cells was measured using ImageJ. ns, not statistically significant. c The expression of GRA3Wh6 was confirmed by immunoblotting. Molecular weight (MW) of GFP = 28k Da, MW of pEGFP-GRA3Wh6 fusion protein = 58 kDa

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Parasites & Vectors

ISSN: 1756-3305

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