Fig. 4

Expression of apoptosis-associated proteins and Endoplasmic reticulum stress (ERS) proteins induced by GRA3_wh6 N2a cells were transfected with either pEGFP or pEGFP-GRA3_Wh6 (GRA3_Wh6) for a 24 h period. The expression levels of ER stress- and apoptosis-associated proteins were then determined by immunoblotting. Mock-transfected cells served as the negative control. The represented values were normalized and expressed relative to β-actin levels. CHOP C/EBP homologous protein, GRP78 78-kDa glucose-regulated protein, PERK PKR-like ER kinase, P-PERK phosphorylated PERK. Data were expressed as mean ± SD on three different assays (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001