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Fig. 1 | Parasites & Vectors

Fig. 1

From: Neospora caninum infection induced mitochondrial dysfunction in caprine endometrial epithelial cells via downregulating SIRT1

Fig. 1

Mitochondrial dysfunction in caprine endometrial epithelial cells (EECs) induced by Neospora caninum infection. a, b ROS levels investigated by a DCFH-DA fluorescent probe in caprine EECs infected with N. caninum tachyzoites at a multiplicity of infection (MOI) of 3:1 (parasite:cell) for 48 h. Scale bar, 50 µm. c, d The mitochondrial membrane potential (MMP) detected by JC-1 in caprine EECs at 48 h post infection. Scale bar, 50 µm. e ATP contents detected by an ATP Determination Kit in caprine EECs at 48 h. f Mitochondrial DNA (mtDNA) copy numbers detected by qPCR in caprine EECs at 48 h. g Mitochondrial ultrastructure morphology in caprine EECs detected at 48 h by using transmission electron microscopy (TEM). Scale bar, 1 µm. Data are shown as mean ± standard deviation (SD) of three independent experiments. P-values were calculated using Student’s t test. **P < 0.01; ***P < 0.001

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