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Fig. 2 | Parasites & Vectors

Fig. 2

From: Neospora caninum infection induced mitochondrial dysfunction in caprine endometrial epithelial cells via downregulating SIRT1

Fig. 2

Expression analysis of Sirtuin 1 (SIRT1) in caprine endometrial epithelial cells (EECs) infected with Neospora caninum. a The mRNA levels of SIRT1 determined by RT-qPCR. The protein levels of SIRT1 determined by western blotting. RT-qPCR (a) and western blotting (b–e) were performed to detect the expression of SIRT1 in caprine EECs infected with N. caninum tachyzoites at a multiplicity of infection (MOI) of 3:1 (parasite:cell) for 48 h. Additionally, the protein levels were determined in caprine EECs pre-treated with 50 μM resveratrol (RSV) (b, c) or 20 μM Ex 527 (d, e) for 1 h and then infected with N. caninum tachyzoites at MOI of 3:1 (parasite:cell) for 48 h. Data are shown as mean ± standard deviation (SD) of three independent experiments. P-values were calculated using Student’s t test. *P < 0.05; **P < 0.01; ***P < 0.001

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