Fig. 4

Effect of Sirtuin 1 (SIRT1) on Neospora caninum-induced autophagy in caprine endometrial epithelial cells (EECs). a–d The protein levels of LC-3II determined by Western blotting. e–h The protein levels of p62 determined by western blotting. Caprine EECs were pre-treated with 50 μM resveratrol (RSV) (a, b, e, f) or 20 μM Ex 527 (c, d, g, h) for 1 h and then infected with N. caninum tachyzoites at a multiplicity of infection (MOI) of 3:1 (parasite:cell) for 48 h. Data are shown as mean ± standard deviation (SD) of three independent experiments. P-values were calculated using Student’s t test. *P < 0.05. NS no significant difference was observed