Fig. 5

Intracellular propagation of Neospora caninum tachyzoites in caprine endometrial epithelial cells (EECs). Caprine EECs were pre-treated with 10, 20 and 50 μM resveratrol (RSV, a, b) or 5, 10 or 20 µM Ex 527 (c, d) for 1 h and then infected with N. caninum tachyzoites at a multiplicity of infection (MOI) of 3:1 (parasite:cell) for 48 h. The numbers of N. caninum tachyzoites of vacuoles were counted by randomly selecting 100 parasitophorous vacuoles. Three independent experiments were conducted in triplicate. Data are shown as mean ± standard deviation (SD) of three independent experiments. P-values were calculated using Student’s t test. *P < 0.05. NS no significant difference was observed