Fig. 2

Overall urine metabolic profile during T. gondii infection. Principal component analysis (PCA) score scatter plots of identified metabolites obtained from UHPLC-MS/MS fingerprints in ESI + a or ESI − mode c. Unsupervised hierarchical clustering of metabolic profiling data in ESI + b or ESI − mode d. Metabolite intensity is normalized so that blue represents low intensity, and reddish brown represents high intensity. Columns were hierarchically clustered based on an average linkage using Pearson correlation coefficients as the distance measure. Sample groups including acutely infected, chronically infected, and healthy controls are labeled as AI, CI and Con, respectively