Fig. 4

Quantitative analysis of mouse urinary metabolites in response to T. gondii infection in ESI + mode. a Volcano plot of quantified metabolites in acutely infected vs. control group, chronically infected vs. control group and acutely infected vs. chronically infected group. In this plot, the x-axis is log2 fold-change, which shows the direction of the change (negative scale is decrease and positive scale is increase) in the levels of metabolite intensity, while the y-axis is the -log10 P-value, which shows the significance of the change. b Comparison of significantly changed metabolites between acutely infected, chronically infected and control mice in ESI + mode. Each row represents data for a particular metabolite, and each column represents a urine sample. The colors reddish brown and blue reflect increasing and reduced metabolite levels, respectively. Columns are hierarchically clustered based on an average linkage using Pearson correlation coefficients as the distance measure. Sample groups including acutely infected, chronically infected and healthy control are labeled as AI, CI and Con, respectively