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Fig. 4 | Parasites & Vectors

Fig. 4

From: MiR-942-5p targeting the IFI27 gene regulates HCT-8 cell apoptosis via a TRAIL-dependent pathway during the early phase of Cryptosporidium parvum infection

Fig. 4

IFI27 is involved in cell apoptosis and parasite eradication following early C. parvum infection via a TRAIL-mediated pathway. A The effect of IFI27 on apoptosis-related molecules in HCT-8 cells. The cells were transfected with pcDNA3.1-IFI27-OE or siRNA-IFI27 for 24 h. Cells were exposed to an equal number of C. parvum sporozoites for up to 48 h, followed by a western blot for TRAIL protein expression and real-time PCR analysis for TRAIL and caspase-8 mRNA. Apoptosis-related molecules were enhanced after transfection with pcDNA3.1-IFI27-OE, but were restrained after transfection with siRNA-IFI27. B, C The effect of rTRAIL on cell apoptosis in HCT-8 cells. Cells were transfected with siRNA-IFI27 for 24 h. The cells were exposed to an equal number of C. parvum sporozoites for 2 h, followed by an incubation with varying concentrations of recombinant TRAIL (10, 25, and 50 ng/ml). After 6 h, the cells were digested and stained, and the apoptosis ratio was assessed by flow cytometry. With the increased concentration, the proportion of apoptosis was gradually increased. All data represent the combined mean ± SD of three independent experiments with two to three technical replicates per experiment. The data were analyzed with a one-way ANOVA followed by a Dunnett’s test for multiple comparisons. *P < 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001

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