Fig. 3

Administration of IL-37 induces polarization of macrophage to M2 phenotype. a Transcript levels for each chemokine tested (listed above each graph) expressed by macrophages were evaluated by quantitative real time-PCR. The messenger RNA levels were expressed relative to the levels in infected model controls following normalization with β-actin. All data are expressed as the mean ± SD, 5 mice per group. Asterisks indicate significant difference at *P < 0.05, **P < 0.01, versus infected model control. b FACS analysis of the ratio of M2 macrophages in the liver egg granuloma of the mice. c FACS analysis of M1 and M2 type macrophages in the liver egg granuloma of the mice. d Statistical analysis of M2 and M1 ratio by FACS analysis. Data represent the mean ± SD from three independent experiments, 5 mice per group. Asterisks indicate significant difference at *P < 0.05, **P < 0.01, ***P < 0.001. Abbreviations: FACS, Fluorescence-activated cell sorting; M1, M2, M1 and M2 macrophages, respectively; NC, Normal mice group; IC, infection model control group