Fig. 12

Determination of apoptosis-related gene expression levels after the knockdown of endogenous bovine HSP60 in T. annulata-transformed cells using qPCR. SiHSP60-1, -2 and -3 are three different SiRNA targeting bovine HSP60. X-axis: 1–3, SiHSP60-1, -2 and -3; Control, SiControl. The bovine β-actin mRNA was used for normalization. Asterisks indicate significant difference at *P < 0.05, **P < 0.01 and ***P < 0.001. SiHSP60: Small interfering RNA against HSP60; qPCR: quantitative PCR; Genes involved in cell apoptosis signaling:  p53, BCL-2, BCL-XL, MCL-1, SURVIVIN, BAX, BAD and cytochrome c