Fig. 9
Expression of bovine HSP60 is related to T. annulata infection. A Analysis of bovine HSP60 expression at the mRNA and protein levels in T. annulata-induced cells and noninfected bovine cells. Control: noninfected bovine cells. B Comparison of bovine HSP60 expression levels between normal and Bup-treated T. annulata-transformed cells using qPCR and immunoblotting. Bup: T. annulata-transformed cells upon Bup treatment; Control: T. annulata-transformed cells; DMSO: T. annulata-transformed cells treated with DMSO. The bovine β-actin mRNA was used for normalization. A mouse anti-α-tubulin monoclonal antibody was used as the loading control. Asterisks indicate significant difference at *P < 0.05,**P < 0.01 and ***P < 0.001. Antiparasitic drug: Buparvaquone (Bup), DMSO: dimethyl sulfoxide; qPCR: quantitative PCR