Fig. 4

T.gHB1 GRA15 activates NF-κB promoter. a T.gHB1 GRA15 expression plasmid was co-transformed with NF-κB-Luc and pTL-TK plasmids into HEK293T cells. After 24 h, the luciferase activity was measured in cell lysates. Data are presented as the values with standard error of three independent experiments. b HEK293T cells were co-transfected with different GRA15 encoding plasmids for 30 h. The expression levels of P65 and phosphor-P65 were detected by Western blotting. c Fluorescence microscopy of p65 in 293 T cells transfected with empty vector (PCMV) or vector for FLAG-tagged GRA15. Hoechst, DNA-intercalating dye. Original magnification. Nuclear translocation of p65 was detected by IFA. d GRA15 expression plasmid was co-transformed with NF-κB-Luc and pTL-TK plasmids into HEK293T cells with increasing amounts of GRA15 plasmid. Data are presented as the mean relative luciferase intensity with standard deviation of three independent experiments (^**P < 0.01; ^***P < 0.001; ^****P < 0.0001; NS not significant)