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Table 2 Overview of primers and probes used for the in-house qPCR screening of three pathogenic agents

From: A community approach for pathogens and their arthropod vectors (ticks and fleas) in cats of sub-Saharan Africa

Target

In-house forward primer

In-house reverse primer

In-house hydrolysis probe

Hepatozoon canis

GGCAGTGACGGTTAACGGGGG

GCACCAGACTTGCCCTCCAATTG

VIC-CCGGAGAGGGAGCCTGAGAAACGG-QSY

Dirofilaria immitis

  

Cy5-CTTTGGAATATGTGTTTTTTTGGAGAGCCCTC-BHQ3

Babesia felis

AAGAAGCTCGTAGTTGAATTTCTGCC

GAGAAGCCGAAGCAACACAAATCCAG

Cy5-TGCGTTTTCCGACTGGCTTGGCA-BHQ3

  1. For all PCRs, the final forward and reverse primer concentrations were 400 nM. The final probe concentration was 200 nM