Skip to main content
Fig. 1 | Parasites & Vectors

Fig. 1

From: Structural analysis of PpSP15 and PsSP9 sand fly salivary proteins designed with a self-cleavable linker as a live vaccine candidate against cutaneous leishmaniasis

Fig. 1

RNA and protein sequences of all studied constructs. The target gene is inserted by stable transfection into the rRNA locus of L. tarentolae. a Schematic presentation of recombinant L. tarentolae rRNA locus. b Schematic presentation of all possible PpSP15/PsSP9 combinations with and without T2A at the mRNA level. Cap (SL sequence), Utr1 spliced sequence, Kozak, and signal sequences were N-terminally inserted in addition to the C-terminal His-tag, stop codon, Utr2 spliced sequence, and poly-A tail. c Schematic presentation of all possible PpSP15/PsSP9 combinations with and without T2A at the protein level. PpSP15 and PsSP9 fusions with the T2A linker give rise to separate individual proteins tagged with C-terminal T2A or six histidines accordingly

Back to article page

Parasites & Vectors

ISSN: 1756-3305

Contact us