Skip to main content

Table 2 Protein validation results for all combinations

From: Structural analysis of PpSP15 and PsSP9 sand fly salivary proteins designed with a self-cleavable linker as a live vaccine candidate against cutaneous leishmaniasis

Construct

Software

ProSA-web

Z-scorea

ERRAT overall quality factorb

Verify3D (%)c

Ramachandran analysisd

Favored

Allowed

Disallowed

PpSP15-T2A-PsSP9

−7.03

80.201%

92.16

74.6%

22.9%

2.5%

PpSP15–PsSP9

−7.49

89.167%

91.94

84.3%

14%

1.7%

PsSP9-T2A-PpSP15

−6.76

60.403

94.44

69.8%

27.4%

2.8%

PsSP9–PpSP15

−7.99

78.75%

95.97

81%

14.8%

4.2%

PpSP15-T2A

−6.56

74.2857

100

88

9

3

PpSP15-Histag

−5.79

94.167

100

92.5%

6.6%

0.8%

PsSP9-T2A

−6.1

93.4783

100

89.6%

8.8%

1.5%

PsSP9-Histag

−5.2

99.145

98.41

95%

4.1%

0.8%

PpSP15

−6.09

99.1304

98.37

87.8%

9.6%

2.6%

PsSP9

−5.83

97.3214

90.83

92.5%

5.8%

0

  1. aIndicates the overall model quality
  2. bThe percentage of the proteins for which the calculated error value falls below 95% rejection limit
  3. cPercentage of amino acids with a 3D/1D score ≥ 0.2
  4. dDihedral angles psi (ψ) versus phi (ϕ) distribution